SrasV1, Main, Exploration, bibRecord, 004E42

Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus

Identifieur interne : 004E42 ( Main/Exploration ); précédent : 004E41; suivant : 004E43

Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus

Auteurs : JINCUN ZHAO [République populaire de Chine] ; WEI WANG [République populaire de Chine] ; Guang-Fa Wang [République populaire de Chine] ; YONGHUA LI [République populaire de Chine] ; HUI ZHUANG [République populaire de Chine] ; XIAOYUAN XU [République populaire de Chine] ; FURONG REN [République populaire de Chine] ; ZHENDONG ZHAO [République populaire de Chine] ; Xiao-Ming Gao [République populaire de Chine]

Source :

Journal of clinical virology [ 1386-6532 ] ; 2005.

RBID : Pascal:05-0204185

Descripteurs français

Pascal (Inist)
- Virus syndrome respiratoire aigu sévère, Technique ELISA, Détection, Anticorps, Protéine, Glycoprotéine, Microbiologie, Virologie.

English descriptors

KwdEn :
- Antibody, Detection, ELISA assay, Glycoprotein, Microbiology, Protein, Severe acute respiratory syndrome virus, Virology.

Abstract

Background: Severe acute respiratory syndrome (SARS) is caused by infection with SARS-associated coronavirus (CoV). Amino acid residues 450-650 of the spike (S) glycoprotein of SARS-CoV (S450-650) contains dominant epitopes for anti-viral antibodies (Abs) in patient sera. Objectives: To develop and evaluate an ELISA system for detection of anti-S Abs in patient sera. Study design: Express recombinant S450-650 in E. Coli and evaluate the sensitivity and specificity of an ELISA system based on the S450-650 polypeptide. Results: The S450-650-based ELISA detected IgG Abs in 41 out of 51 serum samples from 22 hospitalized patients with probable SARS, a result closely correlated with that obtained with a virus-based ELISA (r=0.75, k=0.8). Differential anti-S IgG responses were observed amongst SARS patients. Some of them produced anti-S Abs early during their infection, while others failed to make IgG Abs against the S450-650 polypeptide. None of the serum samples from 100 healthy blood donors was positive in the S450-650-based assay. Conclusion: The S450-650-based ELISA can detect anti-S IgG Abs with high sensitivity and specificity.

Affiliations:

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Le document en format XML

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibody</term>
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<term>Severe acute respiratory syndrome virus</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Virus syndrome respiratoire aigu sévère</term>
<term>Technique ELISA</term>
<term>Détection</term>
<term>Anticorps</term>
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<front><div type="abstract" xml:lang="en">Background: Severe acute respiratory syndrome (SARS) is caused by infection with SARS-associated coronavirus (CoV). Amino acid residues 450-650 of the spike (S) glycoprotein of SARS-CoV (S450-650) contains dominant epitopes for anti-viral antibodies (Abs) in patient sera. Objectives: To develop and evaluate an ELISA system for detection of anti-S Abs in patient sera. Study design: Express recombinant S450-650 in E. Coli and evaluate the sensitivity and specificity of an ELISA system based on the S450-650 polypeptide. Results: The S450-650-based ELISA detected IgG Abs in 41 out of 51 serum samples from 22 hospitalized patients with probable SARS, a result closely correlated with that obtained with a virus-based ELISA (r=0.75, k=0.8). Differential anti-S IgG responses were observed amongst SARS patients. Some of them produced anti-S Abs early during their infection, while others failed to make IgG Abs against the S450-650 polypeptide. None of the serum samples from 100 healthy blood donors was positive in the S450-650-based assay. Conclusion: The S450-650-based ELISA can detect anti-S IgG Abs with high sensitivity and specificity.</div>
</front>
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<affiliations><list><country><li>République populaire de Chine</li>
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<name sortKey="Furong Ren" sort="Furong Ren" uniqKey="Furong Ren" last="Furong Ren">FURONG REN</name>
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<name sortKey="Hui Zhuang" sort="Hui Zhuang" uniqKey="Hui Zhuang" last="Hui Zhuang">HUI ZHUANG</name>
<name sortKey="Wang, Guang Fa" sort="Wang, Guang Fa" uniqKey="Wang G" first="Guang-Fa" last="Wang">Guang-Fa Wang</name>
<name sortKey="Wei Wang" sort="Wei Wang" uniqKey="Wei Wang" last="Wei Wang">WEI WANG</name>
<name sortKey="Xiaoyuan Xu" sort="Xiaoyuan Xu" uniqKey="Xiaoyuan Xu" last="Xiaoyuan Xu">XIAOYUAN XU</name>
<name sortKey="Yonghua Li" sort="Yonghua Li" uniqKey="Yonghua Li" last="Yonghua Li">YONGHUA LI</name>
<name sortKey="Zhendong Zhao" sort="Zhendong Zhao" uniqKey="Zhendong Zhao" last="Zhendong Zhao">ZHENDONG ZHAO</name>
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Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus

Development and evaluation of an enzyme-linked immunosorbent assay for detection of antibodies against the spike protein of SARS-coronavirus

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri